5 mg / kg, dissolved in 2% carboxymethyl cellulose. For experiments, exponentially grown cells were harvested by centrifugation and resuspended in fresh medium containing 10% FBS.p.5 times; 10 5 cells per well, and serum-starved for 24 hours in DMEM only.
They are for reference only..Rapamycin is a specific mTOR inhibitor with IC50 of 0. In Vivo: Rapamycin (i.3 mL of fresh medium was added to each well. They are for reference only. Cell Assay: HL-60, NB4, U937, KG-1 and K562 cells are routinely passaged in RPMI-1640, supplemented with 10% heat-inactivated pcr tube and plate FBS, 2 mM L-glutamine, 50 U / mL penicillin and 50 mu g / mL streptomycin. An atmosphere humidified with 5% CO2 at 37 deg; C.1nM In Vitro: rapamycin inhibits endogenous mTOR activity in HEK293 cells with IC50 of 0.
The isolated protein was transferred to a PVDF membrane and immunoblotted with a phosphate specific primary antibody against Thr389 of p70 S6 kinase. Rapamycin inhibits cell viability in all three cell lines in a dose-dependent manner, but their sensitivity changes. WT or LS / + mice were treated daily with rapamycin (2 mg / kg body weight, intraperitoneally) for 4 weeks and then injected with the same dose every week for another 4 weeks. The abnormal fetal gene expression profile of rapamycin-treated LS / + mouse hearts was significantly reversed [5]. .5-500nM) or AP21967 (0. Kinase Assay: HEK293 cells were seeded in 12-well plates at 2-2.25% PEG, 0. The IC50 levels of T98G, U87-MG and U373-MG cells were 2 nM, 1 \mu; M and gt; 25 \mu; M [3]. Mice [5] dissolved rapamycin in ethanol at a concentration of 20 mg / mL,
filtered and sterilized, resuspended in a carrier (0. FK506 is delivered once daily by subcutaneous injection at a dose of 3 mg / kg, dissolved in 10% ethanol, 10% cremophor and saline. CsA is delivered once daily by subcutaneous injection at a dose of 15 mg / kg, dissolved in 10% methanol and olive oil. MCE has not independently confirmed the accuracy of these methods.
https://www.mingjibio.com/product/pcr-series/
They are for reference only..Rapamycin is a specific mTOR inhibitor with IC50 of 0. In Vivo: Rapamycin (i.3 mL of fresh medium was added to each well. They are for reference only. Cell Assay: HL-60, NB4, U937, KG-1 and K562 cells are routinely passaged in RPMI-1640, supplemented with 10% heat-inactivated pcr tube and plate FBS, 2 mM L-glutamine, 50 U / mL penicillin and 50 mu g / mL streptomycin. An atmosphere humidified with 5% CO2 at 37 deg; C.1nM In Vitro: rapamycin inhibits endogenous mTOR activity in HEK293 cells with IC50 of 0.
The isolated protein was transferred to a PVDF membrane and immunoblotted with a phosphate specific primary antibody against Thr389 of p70 S6 kinase. Rapamycin inhibits cell viability in all three cell lines in a dose-dependent manner, but their sensitivity changes. WT or LS / + mice were treated daily with rapamycin (2 mg / kg body weight, intraperitoneally) for 4 weeks and then injected with the same dose every week for another 4 weeks. The abnormal fetal gene expression profile of rapamycin-treated LS / + mouse hearts was significantly reversed [5]. .5-500nM) or AP21967 (0. Kinase Assay: HEK293 cells were seeded in 12-well plates at 2-2.25% PEG, 0. The IC50 levels of T98G, U87-MG and U373-MG cells were 2 nM, 1 \mu; M and gt; 25 \mu; M [3]. Mice [5] dissolved rapamycin in ethanol at a concentration of 20 mg / mL,
filtered and sterilized, resuspended in a carrier (0. FK506 is delivered once daily by subcutaneous injection at a dose of 3 mg / kg, dissolved in 10% ethanol, 10% cremophor and saline. CsA is delivered once daily by subcutaneous injection at a dose of 15 mg / kg, dissolved in 10% methanol and olive oil. MCE has not independently confirmed the accuracy of these methods.
https://www.mingjibio.com/product/pcr-series/
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