Part I: Several considerations of subcutaneous tumor-bearing model (1) Selection of tumor cell line The selection of tumor cell line is a very troublesome problem, but it is the most important problem. Generally, 40 mice are injected, and at least 50 mice need to be prepared. According to experience, in general, mouse-derived tumor cells are inoculated with 2 to 1 million per head, while human-derived tumor cells require 200- Above 10 million / piece. Continue to observe whether the tumor cells can grow up after a few weeks. ② Tumor cells are divided into multiple types according to molecular markers. A549. In addition to the tumor formation rate, the experimental purpose must be considered when selecting.
The following takes nude mice as an example to introduce the experimental process of subcutaneous tumor-bearing in detail. In the case where no relevant information is found, the maximum is 10 million / 0. The tumor cells are absorbed by the mice themselves. Therefore, it is generally not used. A brief review is that cell lines and mouse strains are the two most important factors to consider in a subcutaneous tumor-bearing model. Cancer CT26) Part IV: Questions and answers about subcutaneous tumors. ③ The experimental tumor cell lines are mostly human or murine cell lines. Subcutaneous tumor-bearing applications are generally used to screen and detect drugs that inhibit tumor growth (cell proliferation). ⑥
The cells should be kept on ice all the time, so that the cells are in a relatively low metabolic state. Tumor-bearing is a professional term for transplanting tumor cells into mice. Each batch of experiments only uses animals of the same sex, usually male and female, but according to the tumor type and tumor cell line studied (females such as breast cancer, males for prostate cancer, etc. This would have to abort the experiment. Subcutaneous tumors are defined as tumor cell lines transplanted into the skin of the same or heterogeneous animal to form tumor masses.
Therefore, in general, Plastic Micro Tubes characteristics of the cell line should be matched as much as possible, and there should not be too much artificial interference.1ml, which cannot be higher (because the cell suspension has reached a saturated state) And calculate the number of cells in advance. Animals smaller than 4W may be intolerant and die prematurely. ⑤ After the injection, slowly withdraw the needle to avoid leakage
https://www.mingjibio.com/product/centrifuge-tube/
The following takes nude mice as an example to introduce the experimental process of subcutaneous tumor-bearing in detail. In the case where no relevant information is found, the maximum is 10 million / 0. The tumor cells are absorbed by the mice themselves. Therefore, it is generally not used. A brief review is that cell lines and mouse strains are the two most important factors to consider in a subcutaneous tumor-bearing model. Cancer CT26) Part IV: Questions and answers about subcutaneous tumors. ③ The experimental tumor cell lines are mostly human or murine cell lines. Subcutaneous tumor-bearing applications are generally used to screen and detect drugs that inhibit tumor growth (cell proliferation). ⑥
The cells should be kept on ice all the time, so that the cells are in a relatively low metabolic state. Tumor-bearing is a professional term for transplanting tumor cells into mice. Each batch of experiments only uses animals of the same sex, usually male and female, but according to the tumor type and tumor cell line studied (females such as breast cancer, males for prostate cancer, etc. This would have to abort the experiment. Subcutaneous tumors are defined as tumor cell lines transplanted into the skin of the same or heterogeneous animal to form tumor masses.
Therefore, in general, Plastic Micro Tubes characteristics of the cell line should be matched as much as possible, and there should not be too much artificial interference.1ml, which cannot be higher (because the cell suspension has reached a saturated state) And calculate the number of cells in advance. Animals smaller than 4W may be intolerant and die prematurely. ⑤ After the injection, slowly withdraw the needle to avoid leakage
https://www.mingjibio.com/product/centrifuge-tube/
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