5 mg / kg, dissolved in 2% carboxymethyl cellulose. For experiments, exponentially grown cells were harvested by centrifugation and resuspended in fresh medium containing 10% FBS.p.5 times; 10 5 cells per well, and serum-starved for 24 hours in DMEM only.
They are for reference only..Rapamycin is a specific mTOR inhibitor with IC50 of 0. In Vivo: Rapamycin (i.3 mL of fresh medium was added to each well. They are for reference only. Cell Assay: HL-60, NB4, U937, KG-1 and K562 cells are routinely passaged in RPMI-1640, supplemented with 10% heat-inactivated pcr tube and plate FBS, 2 mM L-glutamine, 50 U / mL penicillin and 50 mu g / mL streptomycin. An atmosphere humidified with 5% CO2 at 37 deg; C.1nM In Vitro: rapamycin inhibits endogenous mTOR activity in HEK293 cells with IC50 of 0.
The isolated protein was transferred to a PVDF membrane and immunoblotted with a phosphate specific primary antibody against Thr389 of p70 S6 kinase. Rapamycin inhibits cell viability in all three cell lines in a dose-dependent manner, but their sensitivity changes. WT or LS / + mice were treated daily with rapamycin (2 mg / kg body weight, intraperitoneally) for 4 weeks and then injected with the same dose every week for another 4 weeks. The abnormal fetal gene expression profile of rapamycin-treated LS / + mouse hearts was significantly reversed [5]. .5-500nM) or AP21967 (0. Kinase Assay: HEK293 cells were seeded in 12-well plates at 2-2.25% PEG, 0. The IC50 levels of T98G, U87-MG and U373-MG cells were 2 nM, 1 \mu; M and gt; 25 \mu; M [3]. Mice [5] dissolved rapamycin in ethanol at a concentration of 20 mg / mL,
filtered and sterilized, resuspended in a carrier (0. FK506 is delivered once daily by subcutaneous injection at a dose of 3 mg / kg, dissolved in 10% ethanol, 10% cremophor and saline. CsA is delivered once daily by subcutaneous injection at a dose of 15 mg / kg, dissolved in 10% methanol and olive oil. MCE has not independently confirmed the accuracy of these methods.
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They are for reference only..Rapamycin is a specific mTOR inhibitor with IC50 of 0. In Vivo: Rapamycin (i.3 mL of fresh medium was added to each well. They are for reference only. Cell Assay: HL-60, NB4, U937, KG-1 and K562 cells are routinely passaged in RPMI-1640, supplemented with 10% heat-inactivated pcr tube and plate FBS, 2 mM L-glutamine, 50 U / mL penicillin and 50 mu g / mL streptomycin. An atmosphere humidified with 5% CO2 at 37 deg; C.1nM In Vitro: rapamycin inhibits endogenous mTOR activity in HEK293 cells with IC50 of 0.
The isolated protein was transferred to a PVDF membrane and immunoblotted with a phosphate specific primary antibody against Thr389 of p70 S6 kinase. Rapamycin inhibits cell viability in all three cell lines in a dose-dependent manner, but their sensitivity changes. WT or LS / + mice were treated daily with rapamycin (2 mg / kg body weight, intraperitoneally) for 4 weeks and then injected with the same dose every week for another 4 weeks. The abnormal fetal gene expression profile of rapamycin-treated LS / + mouse hearts was significantly reversed [5]. .5-500nM) or AP21967 (0. Kinase Assay: HEK293 cells were seeded in 12-well plates at 2-2.25% PEG, 0. The IC50 levels of T98G, U87-MG and U373-MG cells were 2 nM, 1 \mu; M and gt; 25 \mu; M [3]. Mice [5] dissolved rapamycin in ethanol at a concentration of 20 mg / mL,
filtered and sterilized, resuspended in a carrier (0. FK506 is delivered once daily by subcutaneous injection at a dose of 3 mg / kg, dissolved in 10% ethanol, 10% cremophor and saline. CsA is delivered once daily by subcutaneous injection at a dose of 15 mg / kg, dissolved in 10% methanol and olive oil. MCE has not independently confirmed the accuracy of these methods.
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The freezing PCR Tubes And Plates of the freezer is generally below -20 ℃, which can be used for the storage of bacteria and some reagents. Its types are portable, vertical, horizontal, etc. According to the needs of use, the laboratory can set special incubators for different temperatures, such as 37 ° C, 30 ° C, 25 ° C and so on. Second, to ensure the sterilization (sterilization) of laboratory supplies and equipment for testing in the food microbiology laboratory, the equipment used for sterilization is usually a high-pressure steam sterilizer or a dry box for dry heat sterilization., Ltd.
Among them, the homogenizer can be divided into a flapping homogenizer and a rotary vane homogenizer. Due to the wide application of electronic balances, the use of tray balances has gradually decreased. Drying box is mainly used for dry heat sterilization of metal and glassware. Product. Pallet balances are often used in applications where the amount of symmetry is not critical.Common instruments and equipment in the microbiology testing laboratory The instruments and equipment in the laboratory refer to the instruments and equipment set up for teaching, scientific research, and technical development tests., Ltd. Usually people are used to call the instruments and equipment commonly used for testing, and the larger instruments and equipment used for production and production are called equipment.
If the existing biochemical identification items are not improved based on the new types added, simply increasing the classification entries in the database will not improve the accuracy of the identification system. The cost of improving database entries while adding database entries is very high. Manufacturers of certification systems must then keep up and make adjustments accordingly. In view of the above circumstances, it is difficult for users to make accurate comparisons and judge the pros and cons of these products. 6. It mainly provides chromatography consumables,
chromatography instruments, solid-phase extraction devices, chemical reagents, sample bottles, deuterium Products and services such as lamps, standards, microbial technology, strain preservation services, and ATCC product agents!. It is one of the requirements to obtain high-quality laboratory test results with instruments and equipment that are suitable for the detection of food microorganisms.
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Among them, the homogenizer can be divided into a flapping homogenizer and a rotary vane homogenizer. Due to the wide application of electronic balances, the use of tray balances has gradually decreased. Drying box is mainly used for dry heat sterilization of metal and glassware. Product. Pallet balances are often used in applications where the amount of symmetry is not critical.Common instruments and equipment in the microbiology testing laboratory The instruments and equipment in the laboratory refer to the instruments and equipment set up for teaching, scientific research, and technical development tests., Ltd. Usually people are used to call the instruments and equipment commonly used for testing, and the larger instruments and equipment used for production and production are called equipment.
If the existing biochemical identification items are not improved based on the new types added, simply increasing the classification entries in the database will not improve the accuracy of the identification system. The cost of improving database entries while adding database entries is very high. Manufacturers of certification systems must then keep up and make adjustments accordingly. In view of the above circumstances, it is difficult for users to make accurate comparisons and judge the pros and cons of these products. 6. It mainly provides chromatography consumables,
chromatography instruments, solid-phase extraction devices, chemical reagents, sample bottles, deuterium Products and services such as lamps, standards, microbial technology, strain preservation services, and ATCC product agents!. It is one of the requirements to obtain high-quality laboratory test results with instruments and equipment that are suitable for the detection of food microorganisms.
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The liquid medium has a uniform distribution of nutrients and is easy to control the growth and metabolism of microorganisms. The medium that is cooled to 55 ~ 60 C is placed in a large Erlenmeyer flask. Therefore, it is only suitable for scientific research, such as nutrition and metabolism. 3) Identification medium Adding a certain reagent or chemical to the culture medium makes the indistinguishable microorganisms show obvious differences after being cultured, thus helping to quickly identify a certain microorganism. Because various microorganisms have different nutritional requirements and different culture purposes and detection needs, there are many types of media.
Micro-sampler (covering 1-1000ul) 2. (3) The Disposable Plastic Ware used for sample processing cannot be used as tools for ordinary molecular cloning, nor can they be used to manipulate target sequences. Experiment to see if the reagents are satisfactory, and then aliquot them into a quantity that is only sufficient for one use for storage. Mobile UV lamp (near the work surface) 5. Micro-sampler (covering 1-1000ul) 3. It is a molecular biology technique used to amplify specific DNA fragments and can be regarded as a special DNA replication outside the organism. ⑸
Large-volume samples should be pipetted using individually packaged sterile disposable pipettes.025% sodium azide to the amplification reagent or sample preparation reagent does not inhibit the amplification reaction. All solutions used should be free of nucleic acid and / or nuclease (DNase and RNase) contamination. The lab coat should be dedicated to the sample preparation room and cleaned frequently. ⑹ Newly prepared reagents should be tested before they are used to prepare new specimens. Gloves should be changed frequently, especially during each step of the extraction process.
The mouth of the container can be closed with a cotton plug with moisture-proof paper, and the outside must be wrapped with waterproof paper (currently test tubes usually have screw caps). It is best to use a stainless steel pan for heating and melting. It is formulated with chemicals of known chemical composition. Bile salts have the ability to inhibit bacteria other than intestinal bacteria (selectivity). 3. 3. Agar, gelatin, silica gel, etc.
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Micro-sampler (covering 1-1000ul) 2. (3) The Disposable Plastic Ware used for sample processing cannot be used as tools for ordinary molecular cloning, nor can they be used to manipulate target sequences. Experiment to see if the reagents are satisfactory, and then aliquot them into a quantity that is only sufficient for one use for storage. Mobile UV lamp (near the work surface) 5. Micro-sampler (covering 1-1000ul) 3. It is a molecular biology technique used to amplify specific DNA fragments and can be regarded as a special DNA replication outside the organism. ⑸
Large-volume samples should be pipetted using individually packaged sterile disposable pipettes.025% sodium azide to the amplification reagent or sample preparation reagent does not inhibit the amplification reaction. All solutions used should be free of nucleic acid and / or nuclease (DNase and RNase) contamination. The lab coat should be dedicated to the sample preparation room and cleaned frequently. ⑹ Newly prepared reagents should be tested before they are used to prepare new specimens. Gloves should be changed frequently, especially during each step of the extraction process.
The mouth of the container can be closed with a cotton plug with moisture-proof paper, and the outside must be wrapped with waterproof paper (currently test tubes usually have screw caps). It is best to use a stainless steel pan for heating and melting. It is formulated with chemicals of known chemical composition. Bile salts have the ability to inhibit bacteria other than intestinal bacteria (selectivity). 3. 3. Agar, gelatin, silica gel, etc.
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First put a section of latex tube with a length of 8 ~ 10cm on the mouth of the glass funnel, lock the stopper, and pour the culture medium into the funnel (the rest of the culture medium should be placed on an electric stove to keep warm). Learn how to make tampons and how to use a portable high-pressure steam cooker. Experimental equipment, tools and materials: Portable pressure cooker, 1000 ～ 1500W electric furnace, 1 / 10g coarse scale,
1000mL measuring cup, one glass funnel, one funnel holder, one 8 ～ 10cm long latex tube, one stop clamp , times; 200mm test tubes, about 150, two wire tubes, a knife and scissors, 250g of ordinary cotton, kraft paper, potato, agar and glucose or sucrose. Sterilize with a portable autoclave. Master the technological process for the preparation of primary culture medium; 3. According to requirements, after adjusting pH with 1% hydrochloric acid or 1% sodium hydroxide solution, aliquot the culture medium into test tubes while hot. When the temperature of the medium drops to about 60 ° C, remove the test tube. (4) tampons. The sterilized oblique interview tube was randomly selected from each group and placed in a 28-32 ° C incubator for 48 to 72 hours.biobw. ⑤ Drain the cold air
Seeing the characteristics of the above products in terms of temperature control technology and safety settings, and a 2-year warranty, maintenance-free for life, is it very tempting? JULABO temperature control equipment is worth possessing by every laboratory researcher.001 ° C, which guarantees the efficient completion of the experiment and prevents the safety hazard caused by temperature overshoot. *
We use RoHS-compliant lab disposable products materials to ensure the protection of the environment and user health.. * The temperature control five-fold safety protection function guarantees the safety and reliability of the equipment itself, prevents the equipment from dry burning, and the remote control can avoid direct operation in a hazardous environment.005 ° C / \plusmn; 0. * Three major temperature control features of ICC / TCF / ATC ensure the accuracy and stability of temperature control, up to \ plusmn; 0. * The three concepts of PRESTO / SMART PUMP / ACC quickly deal with the danger of sudden heat release in experiments
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1000mL measuring cup, one glass funnel, one funnel holder, one 8 ～ 10cm long latex tube, one stop clamp , times; 200mm test tubes, about 150, two wire tubes, a knife and scissors, 250g of ordinary cotton, kraft paper, potato, agar and glucose or sucrose. Sterilize with a portable autoclave. Master the technological process for the preparation of primary culture medium; 3. According to requirements, after adjusting pH with 1% hydrochloric acid or 1% sodium hydroxide solution, aliquot the culture medium into test tubes while hot. When the temperature of the medium drops to about 60 ° C, remove the test tube. (4) tampons. The sterilized oblique interview tube was randomly selected from each group and placed in a 28-32 ° C incubator for 48 to 72 hours.biobw. ⑤ Drain the cold air
Seeing the characteristics of the above products in terms of temperature control technology and safety settings, and a 2-year warranty, maintenance-free for life, is it very tempting? JULABO temperature control equipment is worth possessing by every laboratory researcher.001 ° C, which guarantees the efficient completion of the experiment and prevents the safety hazard caused by temperature overshoot. *
We use RoHS-compliant lab disposable products materials to ensure the protection of the environment and user health.. * The temperature control five-fold safety protection function guarantees the safety and reliability of the equipment itself, prevents the equipment from dry burning, and the remote control can avoid direct operation in a hazardous environment.005 ° C / \plusmn; 0. * Three major temperature control features of ICC / TCF / ATC ensure the accuracy and stability of temperature control, up to \ plusmn; 0. * The three concepts of PRESTO / SMART PUMP / ACC quickly deal with the danger of sudden heat release in experiments
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Part I: Several considerations of subcutaneous tumor-bearing model (1) Selection of tumor cell line The selection of tumor cell line is a very troublesome problem, but it is the most important problem. Generally, 40 mice are injected, and at least 50 mice need to be prepared. According to experience, in general, mouse-derived tumor cells are inoculated with 2 to 1 million per head, while human-derived tumor cells require 200- Above 10 million / piece. Continue to observe whether the tumor cells can grow up after a few weeks. ② Tumor cells are divided into multiple types according to molecular markers. A549. In addition to the tumor formation rate, the experimental purpose must be considered when selecting.
The following takes nude mice as an example to introduce the experimental process of subcutaneous tumor-bearing in detail. In the case where no relevant information is found, the maximum is 10 million / 0. The tumor cells are absorbed by the mice themselves. Therefore, it is generally not used. A brief review is that cell lines and mouse strains are the two most important factors to consider in a subcutaneous tumor-bearing model. Cancer CT26) Part IV: Questions and answers about subcutaneous tumors. ③ The experimental tumor cell lines are mostly human or murine cell lines. Subcutaneous tumor-bearing applications are generally used to screen and detect drugs that inhibit tumor growth (cell proliferation). ⑥
The cells should be kept on ice all the time, so that the cells are in a relatively low metabolic state. Tumor-bearing is a professional term for transplanting tumor cells into mice. Each batch of experiments only uses animals of the same sex, usually male and female, but according to the tumor type and tumor cell line studied (females such as breast cancer, males for prostate cancer, etc. This would have to abort the experiment. Subcutaneous tumors are defined as tumor cell lines transplanted into the skin of the same or heterogeneous animal to form tumor masses.
Therefore, in general, Plastic Micro Tubes characteristics of the cell line should be matched as much as possible, and there should not be too much artificial interference.1ml, which cannot be higher (because the cell suspension has reached a saturated state) And calculate the number of cells in advance. Animals smaller than 4W may be intolerant and die prematurely. ⑤ After the injection, slowly withdraw the needle to avoid leakage
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The following takes nude mice as an example to introduce the experimental process of subcutaneous tumor-bearing in detail. In the case where no relevant information is found, the maximum is 10 million / 0. The tumor cells are absorbed by the mice themselves. Therefore, it is generally not used. A brief review is that cell lines and mouse strains are the two most important factors to consider in a subcutaneous tumor-bearing model. Cancer CT26) Part IV: Questions and answers about subcutaneous tumors. ③ The experimental tumor cell lines are mostly human or murine cell lines. Subcutaneous tumor-bearing applications are generally used to screen and detect drugs that inhibit tumor growth (cell proliferation). ⑥
The cells should be kept on ice all the time, so that the cells are in a relatively low metabolic state. Tumor-bearing is a professional term for transplanting tumor cells into mice. Each batch of experiments only uses animals of the same sex, usually male and female, but according to the tumor type and tumor cell line studied (females such as breast cancer, males for prostate cancer, etc. This would have to abort the experiment. Subcutaneous tumors are defined as tumor cell lines transplanted into the skin of the same or heterogeneous animal to form tumor masses.
Therefore, in general, Plastic Micro Tubes characteristics of the cell line should be matched as much as possible, and there should not be too much artificial interference.1ml, which cannot be higher (because the cell suspension has reached a saturated state) And calculate the number of cells in advance. Animals smaller than 4W may be intolerant and die prematurely. ⑤ After the injection, slowly withdraw the needle to avoid leakage
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nuclease-free ultrapure water.china tubes factory, the content of nuclease is less than the detectable range, and the content of microorganisms is less than 0. Cells abuse me thousands of times, and I treat cells like first love.
Tips: Under normal circumstances, the replacement cycle of Lefeng RephiBio terminal filters is 3 months to achieve the best results. Endotoxin-changes the appearance of cells, activates cells, promotes or inhibits cell division, affects cell attachment, etc . Due to the different types of cells and culture conditions, the requirements for the content of impurities in the culture environment are different, so the choice of water for the preparation of culture media or reagents is very particular. 4.
The quality of water in water for in vitro cell culture requires the in vitro cell culture experiment. Water tank level: 0% Or Lü Pure water (ultra-pure water) water quality parameters: resistivity, TOC, temperature terminal water quality real-time monitoring combined with the above water requirements, we recommend two water machines for preparing ultra-pure water, Genie G integrated pure water meter and Genie PURIST ultra pure water meter. Nutrients and metabolites must be dissolved in water to be absorbed and excreted by cells. Water quality requirements for cell culture: 1. Experimental water runs through every link in the whole process of cell culture: 1.
Genie G integrated pure water meter uses tap water as feed water to prepare ultra pure water and EDI secondary pure water performance indicators. It is necessary to maintain the concentration of non-oxygen inert gas such as CO2. For ultrapure water, it is necessary to pay special attention to the TOC, resistivity, bacteria, and endotoxin content of the terminal water quality.; 3. Room ultrapure water. Since the carbon dioxide in the surrounding environment and the air makes it easier for water pollution to change its pH, the container for storing water should be sealed as much as possible to avoid excessive contact with the outside world and inhibit Microbial growth. 3.03EU / mL 3. In order to meet the water quality requirements of cell culture water for the Genie G waterway map, the configuration of the pure water machine is very critical.
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Tips: Under normal circumstances, the replacement cycle of Lefeng RephiBio terminal filters is 3 months to achieve the best results. Endotoxin-changes the appearance of cells, activates cells, promotes or inhibits cell division, affects cell attachment, etc . Due to the different types of cells and culture conditions, the requirements for the content of impurities in the culture environment are different, so the choice of water for the preparation of culture media or reagents is very particular. 4.
The quality of water in water for in vitro cell culture requires the in vitro cell culture experiment. Water tank level: 0% Or Lü Pure water (ultra-pure water) water quality parameters: resistivity, TOC, temperature terminal water quality real-time monitoring combined with the above water requirements, we recommend two water machines for preparing ultra-pure water, Genie G integrated pure water meter and Genie PURIST ultra pure water meter. Nutrients and metabolites must be dissolved in water to be absorbed and excreted by cells. Water quality requirements for cell culture: 1. Experimental water runs through every link in the whole process of cell culture: 1.
Genie G integrated pure water meter uses tap water as feed water to prepare ultra pure water and EDI secondary pure water performance indicators. It is necessary to maintain the concentration of non-oxygen inert gas such as CO2. For ultrapure water, it is necessary to pay special attention to the TOC, resistivity, bacteria, and endotoxin content of the terminal water quality.; 3. Room ultrapure water. Since the carbon dioxide in the surrounding environment and the air makes it easier for water pollution to change its pH, the container for storing water should be sealed as much as possible to avoid excessive contact with the outside world and inhibit Microbial growth. 3.03EU / mL 3. In order to meet the water quality requirements of cell culture water for the Genie G waterway map, the configuration of the pure water machine is very critical.
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Some people say that ’selecting a suitable sample Lab Consumables method is equivalent to half of the analysis work’, which justifies the importance of sample pre-treatment. For sample preparation, the laboratory should include analytical balances, homogenizers, centrifuges, and thermostated freeze shakers. To extract a sample accurate to 0.01 g from a difficult sample, a high-quality homogenizer is required. Since testing involves multiple sam
ple sizes, the homogenizer should be flexible. Statistical data proves that its linear motion can decompose samples more effectively than horizontal motion, thereby improving the extraction rate and ultimately increasing the number of available samples. High-quality centrifuges are also essential for this testing project. It is used to separate solid particles from liquid in suspension, or to separate two liquids in emulsion that are different in density and incompatible with each other. It is an important experimental step for sample preparation. OHAUS provides high-quality centrifuges with various rotors to meet the centrifugal separation of various samples, and has great flexibility.
As a result, researchers can make repeatable results without worrying about machine wear. The durability of the shaker is also an issue. OHAUS has a variety of shakers with different structures that can withstand temperature fluctuations, including large load circular shakers, mixers and extreme environmental shakers, which can withstand up to 100% humidity. The last thing I want to do during the test is to go back to the laboratory to find a machine failure, or the experiment fails.
This is why reliable laboratory equipment is essential for testing. If you want to learn more about the balance series or other Ohaus products, or are looking for more professional and detailed selection guidance, please dial 4008-217-188 quickly, or click to enter \u0026 ldquo; read the original rdquo; Related information, our professional engineers will be happy to help you!
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ple sizes, the homogenizer should be flexible. Statistical data proves that its linear motion can decompose samples more effectively than horizontal motion, thereby improving the extraction rate and ultimately increasing the number of available samples. High-quality centrifuges are also essential for this testing project. It is used to separate solid particles from liquid in suspension, or to separate two liquids in emulsion that are different in density and incompatible with each other. It is an important experimental step for sample preparation. OHAUS provides high-quality centrifuges with various rotors to meet the centrifugal separation of various samples, and has great flexibility.
As a result, researchers can make repeatable results without worrying about machine wear. The durability of the shaker is also an issue. OHAUS has a variety of shakers with different structures that can withstand temperature fluctuations, including large load circular shakers, mixers and extreme environmental shakers, which can withstand up to 100% humidity. The last thing I want to do during the test is to go back to the laboratory to find a machine failure, or the experiment fails.
This is why reliable laboratory equipment is essential for testing. If you want to learn more about the balance series or other Ohaus products, or are looking for more professional and detailed selection guidance, please dial 4008-217-188 quickly, or click to enter \u0026 ldquo; read the original rdquo; Related information, our professional engineers will be happy to help you!
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